Subheadings: Description of Micrococcus luteus NRRL B-8166 Microscopy . These residential skin organisms are also fairly ubiquitous in the environment. Perkins1, Sarah D., Jennie Mayfield2, and Victoria Fraser3 And. I also had to do the thyoglycate test 3 times to get a conclusive result, further making me skeptical of how active the culture was during the physical tests during week 6, which is where almost all of the inconsistencies arose. Both of these organisms are normal flora of skin and mucous membranes of man and animals. Choose from 82 different sets of biochemical tests identification flashcards on Quizlet. Coagulase •Results: Of those only Vancomycin was closer than 50 millimeters, being 38 millimeters. Nutrient agar with Micrococcus luteus. Under the microscope they are round cells. The antibiotic resistance test showed only minor resistance to the antibiotic Oxacillin, which is likely due to a chance inheritance in the population or complete chance because of the weak strength. To identify our isolate after obtaining the pure culture, we performed many different tests. According to morphological and biochemical characteristics, isolates were identified as Micrococcus luteus and Vibrio sp. Note: All diagnostic methods are not necessarily available in all countries. When looking at the antibiotic test results, the isolate is resistant to none of the applied antibiotics, and is only lightly to intermediately resistant to oxacillin. Muralytic activity of Micrococcus luteus rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation. atmosphere;air contaminant C 7603 C 7603 Micrococcus luteus The National Collection of Type Cultures comprises over 5000 bacterial cultures, over 100 mycoplasmas and more than 500 plasmids, host strains, bacteriophages and transposons. C. Catalase positive bacteria produce gas (O 2) in form av bubbles which shows that the bacterium has a catalase. This was determined since it had no color change. I then transferred the pure culture into a TSB slant to preserve it, keeping it at around 3 degrees Celsius in the lab refrigerator. It is urease and catalase positive. The colony morphology is the same as well, being round, shiny, and sort of flat (Medical Laboratories). MR-VP tests Methy Red (MR) and Voges-Prauskauer (VP) tests are a part of IMViC reactions, which are used in the identification of certain fermentative bacteria (e.g. Most of the bacterium in the gram stains were gram negative, but a significant amount, about twenty percent, showed up as gram positive. The Micrococcus genus is known to be found on dust particles, in water, on skin and skin glands in vertebrates, and some species can be found in milk. 2- M. luteus 3- M.roseus Culture: - Strictly aerobic at 37 C incubation (24 hr) - Grow on ordinary media Nutrient agar - Blood agar and on the Blood agar M. roseus (pink) M. luteus (white) M.varians (yellow) - … Catalase-positive. Oxidase positive • Susceptibility tests 1. when all of these test confirmed then apply above mentioned biochemical tests. Rarely, some organisms are positive for both the tests. “Bacteria Detail.”, The Editors of Encyclopædia Britannica. Then to actually isolate a bacterium, I chose a colony from the initial plate and did a quadrant streak of it to further isolate the bacterium, and then incubated it at 38 degrees Celsius for a week. The colony morphology of being yellow, shiny and smooth line up perfectly with M. luteus (Public Health England). D. Catalase negative bakteria does not have a catalase and therefore oxygene is not formed. It gave a negative result which is conclusive with M. luteus. When looking at the genetic tests, most of the identified strains in the Korona test are Micrococcus luteus. Based on the place of origin of the bacterium that I isolated, I hypothesize that it will either be one of the above bacterium or a similar water loving organism, likely one that also forms a biofilm. Learn biochemical tests identification with free interactive flashcards. Like an animal or a plant, the life of bacteria involves a daily routine of thousands of chemical reactions, many devoted to the breakdown (catabolism) of substrates to extract energy or building materials. Reduction of O2 produces superoxide radicals and hydrogen peroxide, or the hydroxyl radical. Gram-neg chart A … With this almost universal range of survivable living conditions that microbes can live in (particularly bacteria), it would be reasonable to assume that there would be at least one variety living in such a nice, wet, and aerated place as my shower drain. We were aiming to isolate a bacterium from this initial environmental sample by using repeated quadrant streaks to isolate single species colonies, therefore producing a pure culture that we could do a multitude of tests on which include Gram staining, genetic analysis and antibiotic testing along with many other tests. M. luteus is an obligate aerobe (Medical Laboratories). Apply one drop of 3% hydrogene peroxide on a microcscopic slide. PubMed search builder options. “Micrococcus – Public Health Agency of Canada.”, Public Health England. They can even survive in conditions that have very little of even no oxygen, using glycolysis and alternative electron acceptors to create ATP. These tests are performed together because organisms are generally positive for one of them. The results of this analysis are shown in table 1. On MacConkey’s agar, it formed round, opaque and colourless colonies, while on blood agar, it formed cubical packets usually produced colonies with a granular surface and matt appearance. The bacterium also colonizes the human mouth, … When using a fluid thyoglycollate test it resulted in the isolate being a strict aerobe, with all of the bacterium being at the top of the medium where it is oxygenic. The differentiation is based on the detection of oxidase enzyme. Biochemical tests. The Gram stain, while it was gram variable, does not ideally match with the genetic test that resulted in Micrococcus luteus, which can be gram variable but is usually gram positive (Bonjar). Discussion: When looking at all of the results for my isolate, they are not all consistent with Micrococcus luteus. M. luteus has been isolated from the surface of human skin, water and soil 19. I then repeated this three more times to further purify the isolate. When the MSA was inoculated with strains produced pneumonia in a patient with acute leukemia, localized cutaneous infections in immunocompromised patients with HIV-1 disease, and catheter-related infection in patients with pulmonary arterial hypertension. After it was sequenced using an Illumina MiSeq at the UAF Core lab, I used the online program Base Space to analyze the reads that I got from sequencing. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. Catalase positive 2. The microdase test is a rapid method to differentiate Staphylococcus from Micrococcus by detection of the enzyme oxidase. I performed a Gram stain test to identify if the bacterium was gram negative or positive, as described in the Lab 4 handout. Characterization of Micrococcus luteus and Bacillus marisflavi Recovered from Common Dentex (Dentex dentex) Larviculture System. This lines up with M. luteus’ resistances from the tests. Transfer bacteria with a plastic loop to the H 2 O 2 solution. Metabolism, Physiology, and Growth Characteristics of Cocci Metabolism. Micrococcus roseus= pink pigment • Biochemical tests 1. endobj The reactions of the four isolates indicated that they belonged to the species M. Zuteus (Kocur & Martinec, 1972). No immunological or biochemical detection techniques are currently available. “Evaluation of Antibacterial Properties of Iranian Medicinal-Plants against Micrococcus Luteus, Serratia Marcescens, Klebsiella Pneumoniae and Bordetella Bronchoseptica.”. Nonmotile. Thus, M. luteus strains were associated with septic arthritis, prosthetic valve endocarditis, and recurrent bacteremia. <>>> Mukamolova GV, Murzin AG, Salina EG, Demina GR, Kell DB, Kaprelyants AS, Young M. 2006. Few cases have been reported as opportunistic infection or catheter/ shunt related infection. The confidence on that reading is decently confident, evidenced by the blue coloring. Salt-tolerant glutaminases produced by Micrococcus luteus of marine origin are of interest for the production of soy sauces. Also, Micrococcus spp. The challenge tests performed with our strains of Kocuria and Micrococcus on rainbow trout in laboratory aquaria confirmed the three Koch postulates. Security, consultancy, umbrella, recruitment, training, payroll in London. Those are extremely toxic because they are powerful oxidizing agents and destroy cellular constituents very rapidly. It is an aerobic exopolysaccharide producing bacteria that may be found on the skin, mouth and sometimes in the throat of mammals 13,14 . %PDF-1.5 micrococcus luteus biochemical test results. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. A good definitive test for Gram + cocci is the catalase test. %���� An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The rpf gene of Micrococcus luteus encodes an essential secreted growth factor. The catalase test did return positive by bubbling, indicating that it does have the ability to break down the radical hydrogen peroxide into diatomic oxygen and hydrogen. ?��}��=r�u�O�?e�O�?EB��� u�O�&�}���ۤ�~~�p�,�:�H�i}V��}f3�+|ʴ��O��l��UL�&�Ų���I�]-�N�Z�RǻU��gu��$�鼴�#�O�^��*���V�e˜ּ�]���$��ձ��f�^�S�[��q��'u�D�&�a5nz�Lɿ�������N�Y��kF�YG?mxG���o?������g���p���~X�Q7����p������:���HsV�"����Z�F?�dk�i�4���t�QE��OGSiw�#�9��ؙz�����zt������tD�GS�fs�]e�΄��=�H結 Data Table 1: Fermentation … Table 1: Graph from Korona showing the percent reads of each organism, and to which taxonomic level. Biochemical characterization and genetic mapping of purine genes in Micrococcus luteus BY NIRUPAMA MOHAPATR ANAD WESLEY E. KLOOS Department of Genetics, North Carolina State University, Raleigh, North Carolina 27607 {Received 27 May 1975) SUMMARY Purine auxotrophs o Micrococcusf luteus were induced by N-methyl-N'-nitro-IV-nitrosoguanidine. Intro: Microbes are everywhere, even in the most extreme environments and conditions. The colonies are a pale, translucent yellow, and are shiny when looked at in the light. contains peptone, phenol red (a pH indicator), a Durham tube, and one 0 Phenol red is a pH indicator which turns yellow below a the Durham tube. Micrococcus luteus is another gram-positive coccus that also is catalase positive. Auxotrophs were biochemically characterized by examining culture accumulations of histidine intermediates, using paper chromatography and the Bratton-Marshall test, and growth responses to L-histidinol. The API 20 E test strip results revealed nothing about what the isolate uses as an electron acceptor because every result was negative, and that it likely only uses glucose as an energy and carbon source and oxygen as its electron acceptor. Organisms commonly found on the body include Micrococcus, Staphylococcus, and Corynebacterium species (Dermnet New Zeland). Filter paper disks impregnated with tetramethyl-p-phenylenediamine dihydrochloride (oxidase reagent) in dimethyl sulfoxide (DMSO) are used. Micrococcus luteus Micrococcus lylae Micrococcus nishinomiyaensis ... KEY BIOCHEMICAL REACTIONS Urease-variable. Biochemical Tests. Lab 5 (Biochemical Tests in Microbiology) Tables and Questions.docx - Data Table 1 Fermentation in TSI Medium Escherichia coli Staphylococcus. Nutrient agar is the simple medium which uses to grow the bacteria. Modified oxidase test (microdase) is recommended for Gram-positive, catalase-positive cocci, only. Both of these cocci are non-motile, non-sporing and Catalase Positive. micrococcus luteus sim test results Seymour Valentine Services in London | We provides specialist solutions to the public/private sector, commercial and residential markets in UK. Colonies are smooth, … The organism itself is susceptible to almost all drugs, with a few strains being resistant to nitrofurantoin, macrolides and lincomycin (Public Health Canada). I then streaked the exposed swab onto a petri dish to allow the organisms to grow, incubating them at 25 degrees Celsius in a dark cabinet. Another test I did to identify my isolate was DNA analysis, described in lab handouts 5 and 7. Defining characteristics of Micrococcus are the ability to aerobically produce acid from glucose glycerol, aesculin hydrolysis, arginine dihydrolase, major pigment production, motility, and conversion of nitrate to nitrite. They are fairly small as well, usually about a millimeter in diameter and of a normal height. Micrococcus Morphology: - Gram +ve cocci - Arrangement : Tetrades - Non motile, non capsulated, non sporulated Habitat: May be normal present in upper respiratory tract Species : 1-M.varians 2- M. luteus 3- M.roseus Culture: - Strictly aerobic at 37°C incubation (24 hr) - Grow on ordinary media Nutrient agar - Blood agar and It is a gram positive, coccus shaped microbe, and contains catalase. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Year introduced: 1992. Bacitracin sensitive I had a bacterial unknown of M. luteus in my microbiology lab. When looking back at all the data, it is fairly likely that the isolate is Micrococcus luteus, especially when looking at the fluid thyoglycate test, the colony morphology, and the antibiotic susceptibility. Table 1 also indicates the biochemical test results [10–12]. To show the clonal identity of the blood isolates and to separate blood from skin and Broviac catheter M. luteus strains required the development of molecular typing methods. ... Micrococcus luteus Micrococcus roseus Mycobacterium phlei Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Streptococcus pyogenes Streptococcus salivarius. Micrococcus luteus (M. luteus) is a Gram-positive to Gram-variable, non-motile, coccus, saprotrophic bacterium. above mentioned tests are used for confirmation of the Staphylococcus aureus. 2665 ATCC 15307; ATCC 4698; CCM 169; CN 3475; DSM 20030; NCIB 9278; WDCM 00111 Micrococcus luteus They are fairly ubiquitous in the environment, and are small (0.5 to 3.5 micrometers in diameter) and non-motile. It is urease and catalase positive. The Kirby-Bauer disk diffusion … This fits well with where I sampled my bacterium from, as a shower drain is a place where both dust and water would accumulate, along with residues of skin glands from showering. When performing tests for physiological traits, the results were limited. endobj Micrococcus spp. Micrococcus species are oxidase-positive, which can be used to distinguish them from other bacteria like most Staphylococcus species, which are generally oxidase-negative. Table 2: Graph of the genomic reads by taxonomic level using Korona. The test organisms (Lactobacillus plantarum C S and Micrococcus luteus C S) were the recently identified isolates from the corn- soyabean waste-meal. They can be found in They are considered as normal comensal of human skin and upper respiratory tract. M. luteus was first known as Micrococcus lysodeikticus and was discovered by Alexander Fleming in 1928. tetracyclines, β-lactams, a macrolide, and amphenicol. I used the Kirby-Bauer, or disk diffusion test to test for this, described in lab handout 9. Micrococcus luteus Category: In the normal flora Etymology: Genus name: small coccus (seed, grain). STAPHYtest (Lachema, Brno, Czech Republic), a microtiter-based system with nine tests and with eight compartments per isolate for identification of Micrococcus,Stomatococcus, and Staphylococcus, has been described (). <> 2 0 obj Therefore I used this as my location to take my environmental sample from. ... at the genus level using conventional biochemical tests. Catalase test A. Results: Regarding the gram stain test, my isolate was gram variable, I ensured that the strain was fresh so that the age of the culture was the same throughout the test. Micrococcus luteus is a bacterium that belongs to the family Micrococcaceae. Micrococcus luteus= yellow pigment 2. All of the biochemical tests worked well except for the MacConkey agar. pin. Antibacterial disc diffusion studies showed that Kocuria rhizophila and Micrococcus luteus are sensitive to most of the drugs tested i.e. 1. I isolated the DNA by lysing the cell and using the Power Sol DNA Isolation Kit to isolate the DNA from the other parts of the cell. Staphylococcus and Micrococcus both are Gram positive cocci (GPC). January 21, 2014 Acharya Tankeshwar Biochemical tests in Microbiology, Laboratory Diagnosis of Bacterial Disease 6 Bile-esculin test is widely used to differentiate Enterococci and Non-enterococcus group D streptococci, which are bile tolerant and can hydrolyze esculin to esculetin, from non-group D viridans group streptococci, which grow poorly on bile. They are generally strict aerobes and can generally reduce nitrate. Histidine auxotrophs of Micrococcus luteus strain ATCC 27141 were induced by treatment of the parent strain with N-methyl-N'-nitro-N-nitro-soguanidine. 3 0 obj I also hypothesize that it will be an aerobic organism, given that I found it in a well aerated environment and it has survived until I cultured it. M. luteus is a Gram positive cocci (as seen by a gram stain). 1 0 obj When looking back after culturing the isolate, these hypothesies were supported by several of the tests, and served as a good tool to steer me towards what the isolate may be. Species epithet: golden yellow. Staphylococcus in Gram Stain. The oxidase test was negative, as the strip did not change color at all, where it would have turned purple if it was positive. Molecular Microbiology 46(3):611-21. It is devoid of indicator, selective agent, differential ingredients and enriching substances, therefore uses for better expression of pigmentation, biochemical test … Those are extremely toxic because they are powerful oxidizing agents and destroy cellular constituents very rapidly. It is an opportunistic pathogen, only pathogenic enough to cause disease in weakened immune systems (Medical Laboratories). Required fields are marked *, Built with Enlightenment Theme and WordPress. View Lab 5 (Biochemical Tests in Microbiology) Tables and Questions.docx from BIO 275 at Johnston Community College. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. all 3 tests done w/SIM deeps just add Kovac’s reagent for Indole test • Alcaligenes faecalis (left) - ... Micrococcus luteus (left) Alcaligenes faecalis (middle) • Reduction of nitrate to nitrite to be used as a final electron acceptor/Nitrate reductase. M. luteus is the majority of reads on the species level. Secondary habitats for Micrococcus spp. The identified reads only made up a total of twenty seven percent of the total reads, but the majority of those reads were for M. luteus. Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) should be able to repeat your work using only your notebook It can form in tetrads or irregular clusters but not in chains and belongs to the family Micrococcaceae. This microbe forms large, round colonies. Micrococcus luteus It grew in circular, entire, convex and creamy yellow pigmented colonies heaving 0.5-2.5µ diameter. <> atmosphere;air contaminant C 7603 C 7603 Micrococcus luteus The National Collection of Type Cultures comprises over 5000 bacterial cultures, over 100 mycoplasmas and more than 500 plasmids, host strains, bacteriophages and transposons. Reduction of O2 produces superoxide radicals and hydrogen peroxide, or the hydroxyl radical. Significance: [Of minor importance] Old … results clipart test clipart. View Biochemical Tests Lab Report.docx from BIOL 240 at University of Alaska, Anchorage. Oral microbial flora of reptiles includes bacteria from Proteus , Porphyromonas , Micrococcus, Salmonella and Staphylococcus genera 5,8,15 . Agar well diffusion test of 22 selected isolates (Micrococcus spp., n = 8 and S. lentus, n = 14) against the disinfectants showed that, except for Virkon S and Savlon, M. aloeverae was most sensitive to disinfectants, while M. luteus was the least sensitive to all but Virkon S among the three species tested. Acid from glucose was tested with I % (w/v) glucose added aseptically to the Hugh & Leifson oxidation-fermentation medium (Difco) with and without a … Finally, when looking at the API 20E strip none of the results returned positive despite the culture being active and fresh from being streaked recently. <>/ExtGState<>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> If I were to continue researching this isolate, I would redo the API 20 E test strip with a fresh, active culture to ensure that it can reduce nitrate, and also the oxidase test to ensure that it does have cytochrome c oxidase present, which it should according to Public Health England. I would hypothesise that because we clean our shower regularly, that most of the bacterium un the shower drain would come from people in my family taking a shower, and mostly be from the skin. On top of that, most of the bacterium that were stained were gram negative, which conflicts with this result. “Micrococcus.”, h. Shahidi Bonjar. All of these are described in the lab 6 handout and were kept in the incubator at 38 degrees Celsius. Title: FACT SHEET M Luteus Author: User Created Date: 20190118140041Z Phase and light-field microscopy were performed with a LABOROLUX laboratory microscope (Leitz) using a magnification of 700 ×. 4 0 obj Finally I used different antibiotic disks to check for its resistance to antibiotics, using Gentamicin, Cefoperazone, Vancomycin, Tobramycin, Amikacin, Trimethoprim, Oxacillin, and Cefazdin.
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